重組人成纖維細胞生長因子-9（rHuFGF-9）
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- 更新時間： 2024-05-23
- 上一個：重組人成纖維細胞生長因子-22（rHuFGF-22）
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產(chǎn)品介紹
| CYT-415 | 重組人成纖維細胞生長因子-9（rHuFGF-9） | 10μg/50μg/1mg | Prospec |
| Description : | |
| Recombinant Human FGF-9 produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 23 kDa. Recombinant FGF-9 is purified by proprietary chromatographic techniques. The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a single, potential N-linked glycosylation site. The native protein is glycosylated and is efficiently secreted after synthesis, although FGF -9 lacks a typical secretion signal. Rat and mouse FGF-9 show a very high homology to human FGF-9. The transcripts for FGF-9 have been found in brain and in kidney tissue. | |
 | Physical Appearance: |
| Sterile Filtered white lyophilized powder. | |
| Formulation: |
| The sterile protein powder is lyophilized with no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized Recombinant FGF-9 sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized Human FGF-9 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Human FGF-9 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Leu-Gly. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec’s Recombinant FGF-9 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors （measured by 3H-thymidine uptake） is <0.5 ng/ml, corresponding to a specific activity of 5.5 x 106 Units/mg. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of Recombinant Human Fibroblast Growth Factor-9. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.8 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of FGF-9 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美國光譜醫(yī)學透析袋，wako生化試劑，amresco生化試劑，sigma生化試劑，優(yōu)質的分子生物學試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,，洽談選購！
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